Isolation of microtubule protein from cultured mouse neuroblastoma cells.
نویسندگان
چکیده
The addition of vinblastine to high-speed supernatants derived from homogenates of cultured mouse neuroblastoma cells results in the formation of a precipitate which has been characterized as microtubule protein by the following criteria: colchicine-binding activity, molecular weight, amino acid composition, and electrophoretic mobility. The method therefore permits the rapid isolation of microtubule protein from crude supernatants of neuroblastoma cells.
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عنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 65 1 شماره
صفحات -
تاریخ انتشار 1970